Fig. 1.

H₂O₂-activated signaling pathways that regulate CREB protein abundance and CREB-Ser¹³³ phosphorylation in neonatal rat cardiac myocytes. Cell extracts from cardiomyocytes treated with the indicated concentrations of H₂O₂ for 15 or 60 min were subjected to Western blotting with the indicated antibodies. Representative data are depicted in A and B with the results for CREB-Ser¹³³ phosphorylation and CREB, CREMτ2α, CREMα, and ATF-1 protein content after a 60-min treatment with 0.05 mM H₂O₂ quantified in C. In B, the molecular mass markers are applied to all Western blots. *, p < 0.05 versus control (n = 5).