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Lactacystin prevents the H2O2-dependent decrease in CREB protein content. Extracts from cultures pretreated for 30 min with lactacystin (10 μM) and then challenged for 1 h with vehicle or H2O2 (0.05 mM) were subjected to Western blotting for CREB phosphorylation and CREB protein content. Similar results were obtained in two other experiments.
