TABLE 3.

On and off rates and dissociation constants of selective mutants

Values are presented as mean ± S.E.M.

	τon	τoff	koff	kon	kd	n
	s	min	ms−1	μM−1·s−1	μM
WT	7.66 ± 0.72	9.44 ± 1.21	1.77 ± 0.14	51.50 ± 4.83	3.43 ± 0.40	5
Q399C	11.84 ± 0.85*	4.55 ± 0.47*	3.67 ± 0.4*2	32.31 ± 1.24*	11.35 ± 0.69*	5–10
F1236C	8.82 ± 0.91	1.41 ± 0.19*	11.78 ± 0.90*	40.61 ± 3.66	29.02 ± 3.34*	6

^* P < 0.05 versus WT.