Fig. 7.
Induction of oligomerization of hCtr1 in the transfected cells by Cu and CDDP. A, time-dependent induction. SCLC cells were treated with 30 μM CuSO4 for different lengths of time as indicated. Cells were harvested and treated with cross-linking agent BS3. hCtr1 oligomerization was detected by SDS-polyacrylamide gel electrophoresis followed by Western blot. B, concentration-dependent induction of oligomerization. SCLC cells were treated with various concentrations of CuSO4 for 2 h. Oligomerization of hCtr1 was analyzed. C, time-dependent induction of hCtr1 oligomerization by CDDP in various transfected cells. D, time-dependent induction of hCtr1 oligomerization by copper in different transfected cells. E, induction of hCtr1 oligomerization by copper (top) and by CDDP (bottom) in various mutant hCtr1-transfected cells. Monomeric to trimeric species are denoted by one to three black ovals, respectively.