TABLE 2.
Copper |
Cisplatin |
|||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Km | KmMutant/Vector | P | Vmax | Vmax Mutant/Vector | P | Km | KmMutant/Vector | P | Vmax | Vmax Mutant/Vector | P | |
μM | pmol/mg protein/min | μM | pmol/mg protein/min | |||||||||
SCLC | 4.86 ± 0.5 | 0.95 | 16.2 ± 1.4 | 1.05 | 13.1 ± 1.9 | 1.10 | 22.8 ± 1.9 | 1.02 | ||||
vector | 5.12 ± 0.4 | 15.4 ± 1.4 | 11.9 ± 1.5 | 22.4 ± 1.9 | ||||||||
WT | 5.42 ± 0.4 | 1.06 | 87.2 ± 9.5 | 5.66 | <0.01 | 17.2 ± 2.6 | 1.45 | 117 ± 10 | 5.22 | <0.01 | ||
M43-Q | 3.54 ± 0.3 | 0.69 | <0.01 | 5.24 ± 0.4 | 0.34 | <0.01 | 11.1 ± 1.0 | 0.93 | 8.43 ± 1.3 | 0.38 | <0.05 | |
M45-Q | 3.99 ± 0.3 | 0.78 | <0.05 | 6.99 ± 0.5 | 0.45 | <0.01 | 15.9 ± 1.0 | 1.34 | 8.47 ± 1.0 | 0.38 | <0.05 | |
M154-Q | 3.55 ± 0.3 | 0.69 | <0.01 | 5.02 ± 0.4 | 0.33 | <0.01 | 12.2 ± 1.3 | 1.03 | 11.1 ± 0.5 | 0.50 | <0.05 | |
G167-S | 3.78 ± 0.2 | 0.74 | <0.05 | 6.26 ± 0.5 | 0.41 | <0.01 | 6.82 ± 0.7 | 0.57 | <0.05 | 16.9 ± 0.7 | 0.75 | |
δN2-M154-Q | 5.25 ± 0.4 | 1.03 | 15.5 ± 1.2 | 1.01 | 11.5 ± 1.1 | 0.97 | 21.8 ± 1.6 | 0.97 | ||||
δN2-G167-S | 4.99 ± 0.4 | 0.97 | 14.9 ± 1.1 | 0.97 | 13.7 ± 1.2 | 1.15 | 22.8 ± 0.9 | 1.02 |