FIG. 4.

Single-strand breaks are the predominant subtype of 3MI-induced DNA damage. Four-hour incubation with 5μM 3MI (gray bars) produced a maximal difference in DNA damage levels (average OTM) between pH 7 and 12.1, indicating that the majority of DNA damage in response to 3MI treatment results from single-strand breaks. 0.5% DMSO vehicle control (white bars), positive control for double-strand breaks 5μM doxorubicin (black bars), positive control for single-strand breaks 5μM irinotecan (black and white striped bars), and positive control for alkaline labile sites 200μM ethyl nitrosourea (gray and white checked bars) are also shown. *p < 0.05 compared to vehicle-treated control.