TABLE 1.
Analysis of Cytochrome P450 2F3 3MI- and Benzo[a]pyrene-Mediated Mutations in Salmonella typhimurium TA98
| Treatment | Dose |
Revertants/plate |
||
| μM | Amount/plate | Average | SD | |
| DMSO | ||||
| +NADPH | 5 μl | 28.5 | 0.7 | |
| −NADPH | 5 μl | 22 | 1.4 | |
| 3MI | ||||
| +NADPH | 0.01 | 655 ng | 26 | 8.5 |
| −NADPH | 0.01 | 655 ng | 25.5 | 2.12 |
| +NADPH | 0.1 | 6.5 μg | 65 | 11.3 |
| −NADPH | 0.1 | 6.5 μg | 33 | 1 |
| +NADPH | 1 | 65.5 μg | 80.5 | 2.12 |
| −NADPH | 1 | 65.5 μg | 35 | 4.24 |
| +NADPH | 10 | 655 μg | 86.5 | 3.53 |
| −NADPH | 10 | 655 μg | 27 | 8.48 |
| +NADPH | 25 | 1.64 mg | 49 | 15.5 |
| −NADPH | 25 | 1.64 mg | 22.5 | 2.12 |
| +NADPH | 50 | 3.28 mg | 86.5 | 9.19 |
| −NADPH | 50 | 3.28 mg | 29.5 | 2.12 |
| +NADPH | 100 | 6.56 mg | 125 | 43.8 |
| −NADPH | 100 | 6.56 mg | 32.5 | 16.3 |
| +NADPH | 250 | 16.4 mg | 57.5 | 3.54 |
| −NADPH | 250 | 16.4 mg | 22.5 | 7.78 |
| +NADPH | 500 | 32.8 mg | 63.5 | 6.36 |
| −NADPH | 500 | 32.8 mg | 33 | 7.07 |
| Benzo(a)pyrene | ||||
| +NADPH | 100 | 12.62 mg | 17 | 1 |
| −NADPH | 100 | 12.62 mg | 18 | 4.14 |