FIG. 4.

Suppression by TCDD of LPS-induced cell surface MHC Class II expression. Isolated splenocytes were incubated with FcBlock, labeled with antibodies directed against CD19 and MHC Class II, then fixed with BD Cytofix. Cells were subsequently analyzed by FCM. Populations were gated by first identifying (A) CD19⁺ events, then (B) the individual median fluorescence (MFI) for MHC Class II. Results from six mice per group are depicted as mean MFI ± SE of the mean. ^ap < 0.05 compared with sesame oil + PBS and ^bp < 0.05 compared with sesame oil + LPS.