FIG. 5.

TCDD treatment impaired expression of phenotypic indicators for plasmacytic differentiation. To assess B cells for CD138 expression, isolated splenocytes were incubated with FcBlock, labeled with antibodies directed against CD19 and CD138, then fixed with BD Cytofix. For detection of total Igκ or IgJ, cells were blocked, labeled with CD19 antibodies, and fixed with BD Cytofix. At the completion of the time course, cells were permeabilized with BD Perm/Wash, incubated with antibodies directed against either IgJ or Igκ and analyzed by FCM. Populations were gated by first identifying CD19⁺ events as depicted for (A) CD138 and (B) Igκ, and then gated on populations that expressed elevated levels of (C) CD138, (D) Igκ, or (E) IgJ. Results from 6 mice per group are depicted as mean frequency of CD19⁺ cells ± SE of the mean for respective measurements. ^ap < 0.05 compared with sesame oil + PBS and ^bp < 0.05 compared with sesame oil + LPS.