Table 1.

Thermodynamic and kinetic parameters for different tRNA_GGC^Ala on cognate and near-cognate codons

	Kd (nM)a		kGTPmax (s−1)b		kpep (s−1)a
Codon	A-U	U-A	A-U	U-A	A-U	U-A	C-G	C-A
GGC	2.3 ± 0.40	1.0 ± 0.23	31 ± 13	27 ± 11	1.7 ± 0.21	1.8 ± 0.69	1.5 ± 0.70	1.4 ± 0.27
GCU	5.9 ± 0.93	1.3 ± 0.24	23 ± 3.3	25 ± 3.1	1.4 ± 0.17	1.4 ± 0.16	1.5 ± 0.36	1.8 ± 0.15
GCG	~1000c	175 ± 30
GCA	~1000c	210 ± 52	2.4 ± 0.23	26 ± 18	<0.05d	2.1 ± 0.34	<0.05 d	3.4 ± 0.64
GUC					<0.05 d	1.4 ± 0.36	<0.05 d	1.6 ± 0.35
ACC					<0.05 d	0.49 ± 0.16	<0.05 d	2.8 ± 0.79

Error values are the standard error of the mean value.

^aValues are the average of at least three independent experiments.

^bValues were determined based on curves fit to at least four apparent k_GTP values determined at different ribosome concentrations.

^cEstimated value since precise K_d determination exceeded the limits of accurate measurement.

^dEstimated limit (see Methods).