Figure 1.

GluN1^Rneo/+ mice express NMDAR GluN1^R subunits at low levels. (A) Genomic organization of the GluN1^Rneo allele with neo cassette in intron 18. Arrows indicate PCR primers for mRNA quantification plaque assay. Splicing that leads to the GluN1^R hypomorph is indicated for the PCR fragment. (B) Western blot from P0 brain membranes, probed with antibody against GluN1 C terminus. The weak GluN1^Rneo/Rneo signal is comparable to that obtained from GluN1^−/− preparations spiked with 5% GluN1^+/+ material (sample-loading control: ErbB-4). (C) Relative GluN1^R mRNA abundance in forebrain of adult GluN1^Rneo/+ mice (n = 5), determined by a plaque assay. (D) Western blot on adult brain homogenate. Antibodies against GluN1 N or C terminus revealed full-length GluN1 subunits and no truncated species in GluN1^Rneo/+. (E) Relative GluN1^R mRNA abundance of GluN1^R transcripts from individual CA1 pyramidal neurons or DG granule cells (n = 7 cells, each region), determined by plaque assay.