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. 2009 Aug 21;9:173. doi: 10.1186/1471-2180-9-173

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Copyright ©2009 Chagué et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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cgopt1-silenced mutants exhibit reduced pigmentation and sporulation. A. Wild-type, Ori51 and Ori83 strains were cultured on REG plates. Picture was taken after 8 days. B. Sporulation assay: Ori51 (black bars) and wild-type (empty bars) strains were cultured on EMS agar medium in 90-mm Petri dishes. Spores were harvested and counted after 5 days. Data from one experiment are presented. Bars are the mean and SD of five replications. Differences between wild type and the mutant were found significant according to t-test analysis (P < 0.05) in each of the time points (days 4, 6, 8, and 10). Repetition of experiments led to similar results.