Figure 3.

Annexin V-FITC assay. A) normal live Giardia cells, B) Giardia cells treated with H₂O₂ for 2 h. C) Giardia cells treated with protease inhibitor cocktail and then further treated with H₂O₂ for 2 h. D) Giardia cells treated with metronidazole for 4 h. E) Giardia cells treated with protease inhibitor cocktail and then further treated with metronidazole for 4 h. F) Giardia cells grown in modified medium deprived of Cys-ascorbate for 6 h. G) Giardia cells grown in modified medium deprived of Cys-ascorbate with protease inhibitor cocktail. Panels B to G clearly show ring formation due to apoptosis.