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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: Cancer Res. 2008 Jun 1;68(11):4269–4276. doi: 10.1158/0008-5472.CAN-07-5950

Figure 4. The cancer predisposing mutation R1699W disrupts the association of BRCA1 with RAP80.

Figure 4

HEK293T cells were transfected with pLXIN-3×FLAG-RAP80 and pcDNA3-Myc-BRCA1 or mutant pcDNA3-Myc-BRCA1(R1699W) (A) as indicated. Or cells were transfected with pcDNA3-Myc-BRCA1 and pLXIN-3×FLAG-RAP80 or one of the RAP80 mutants, pLXIN-3×FLAG-RAP80(T373S), (T373A), or (F376W) (B) as indicated. Forty-eight h after transfection, cell lysates were prepared and FLAG-RAP80 protein complexes isolated with an anti-FLAG resin. Complexes were then examined by Western blot analysis with antibodies against Myc and FLAG.