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Chemoenzymatic synthesis of STn antigen analogs using a one-pot three-enzyme system. a) 3-chloropropanol, acetyl chloride, 70 °C, 87%; b) NaN3, NaI, DMF, 60 °C, 95%; c) 3 (20 mM), 4, 5, 6, or 7 (30 mM), pyruvate (150 mM), CTP (30 mM), MgCl2 (20 mM), a sialic acid aldolase, a CMP-sialic acid synthetase, Pd2,6ST, Tris-HCl (100 mM, pH 8.5 for 4 and 5; pH 7.5 for 6 and 7).
