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Conjugation of STn antigens to human serum albumin (HSA) using adipic acid p-nitrophenyl diester coupling reagent. a) Pd/C, H2, H2O; b) adipic acid p-nitrophenyl diester, DMF, rt, > 90%; c) phosphate buffer (100 mM, pH 7.4). Average numbers of sialoside molecules incorporated were determined by MAlDI-TOF.
