Table 1.
DNA sequence variations of HPV16 variants from nucleotide position 7722 to 567 detected by polymerase chain reaction-based cloning and sequencing
| Nucleotide alterations at position* |
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 7 | 7 | 7 | 7 | 7 | 7 | 7 | 3 | 9 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 4 | ||||
| 7 | 7 | 8 | 8 | 8 | 8 | 8 | 1 | 0 | 0 | 3 | 4 | 4 | 8 | 8 | 3 | 0 | ||||
| 6 | 8 | 2 | 3 | 3 | 3 | 6 | 9 | 2 | 3 | 5 | 6 | 9 | 5 | 3 | ||||||
| 4 | 6 | 6 | 4 | 7 | 9 | 8 | ||||||||||||||
| Sample | No. of clones | C | C | G | G | A | A | G | C | A | T | G | C | G | T | A | C | A | HPV16 Prototype |
|
| Initial test | Enrollment | 8 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | E variant |
| Enrollment | 1 | T | T | A | T | C | G | A | - | - | - | - | - | - | - | - | - | - | Recombinant 1 | |
| Enrollment | 1 | - | - | - | - | - | - | - | T | T | C | T | G | T | - | - | - | - | Recombinant 2 | |
| Follow-up | 10 | T | T | A | T | C | G | A | T | T | C | T | G | T | A | G | T | G | Af2 variant | |
| Retest | Enrollment | 8 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | E variant |
| Enrollment | 1 | T | T | A | T | C | G | A | T | T | C | T | G | T | - | - | - | - | Recombinant 3 | |
| Enrollment | 1 | - | - | - | - | - | - | - | - | T | C | T | G | T | A | G | T | G | Recombinant 4 | |
| Follow-up | 10 | T | T | A | T | C | G | A | T | T | C | T | G | T | A | G | T | G | Af2 variant | |
*
The nucleotide positions are numbered according to those documented in Human Papillomavirus 1997 Compendium (Meissner, 1997) and correspond to those of the original HPV16 sequence (Seedorf et al., 1985). For each variant sequence, positions that are identical to the prototype sequence are marked with a dash in the alignment. HPV = human papillomavirus; E = European; Af2 = African 2.