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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Clin Cancer Res. 2009 Sep 29;15(19):6208–6216. doi: 10.1158/1078-0432.CCR-09-0592

Fig. 3 — A) Location of the ODC1 promoter SNP. The SNP under investigation in this study is 316 nucleotides 3′ of the ODC1 transcription start site (marked with an asterisk). This SNP resides between two consensus E-boxes as shown by the underlined sequences, and affects a PstI restriction site, marked with a box. B) RFLP analysis of ODC1 SNP. DNA was obtained from two cell types, and the region surrounding the ODC1 SNP site was sequenced. Colon-derived HT29 cells were found to be heterozygous GA, while HCT116 cells were found to be homozygous GG, at the ODC1 SNP locus. A 350 bp PCR product of this region was obtained from each cell type and subjected to digestion with PstI. Evidence of an A-allele was indicated by restriction products smaller than 350 bp.

Fig. 3 — A) Location of the ODC1 promoter SNP. The SNP under investigation in this study is 316 nucleotides 3′ of the ODC1 transcription start site (marked with an asterisk). This SNP resides between two consensus E-boxes as shown by the underlined sequences, and affects a PstI restriction site, marked with a box. B) RFLP analysis of ODC1 SNP. DNA was obtained from two cell types, and the region surrounding the ODC1 SNP site was sequenced. Colon-derived HT29 cells were found to be heterozygous GA, while HCT116 cells were found to be homozygous GG, at the ODC1 SNP locus. A 350 bp PCR product of this region was obtained from each cell type and subjected to digestion with PstI. Evidence of an A-allele was indicated by restriction products smaller than 350 bp.