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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Clin Cancer Res. 2009 Sep 29;15(19):6208–6216. doi: 10.1158/1078-0432.CCR-09-0592

Fig. 4.

Fig. 4

A) E-box protein expression in colon-derived cells. Expression of proteins to be evaluated for binding to the +316 ODC1 SNP was assessed by Western blot analysis. Extracts of both HT29 and HCT116 cells were evaluated for c-MYC, MAD1 and MAD4; β-actin was used as a loading control. B) Documentation of allele-specific transcription factor binding by chromatin immunoprecipitation (CHIP) analysis. CHIP analysis was conducted as described in Material and Methods. HT29 cells were a source of ODC1 A-alleles, as these cells are heterozygous GA at this site. HCT116 cells were used as a source of ODC1 G-alleles.