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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Clin Cancer Res. 2009 Sep 29;15(19):6208–6216. doi: 10.1158/1078-0432.CCR-09-0592

Fig. 5.

Fig. 5

A) Effect of c-MYC expression on ODC1 allele-specific promoter activity in HT29 colon derived cells. Promoter activity was measured after transfection with ODC1 promoter reporter plasmids co-transfected with pcDNA 3.0 plasmid (□) or CMV-MYC expression vector (■). Promoter constructs differ by the presence of the first E-box element, located in −485 to −480 bp (“wt E-box1” for the wild-type sequence or “mut E-box1”, for a mutant sequence). The constructs differ also by the ODC1 +316 SNP (“+316 G” or “+316 A”). *, P ≤ 0.013 for each of the four comparisons relative to promoter activity with pcDNA 3.0 co-transfection. B) Effect of MAD1 expression on ODC1 allele-specific promoter activity in HT29 colon tumor derived cells. Promoter activity was measured after transfection with ODC1 promoter reporter plasmids co-transfected with pcDNA 3.1 plasmid (□) or with a pcDNA-MAD1 plasmid (■). Promoter constructs used were described in the legend for panel A of this figure. *, P = 0.027, statistical significance relative to promoter activity with pcDNA 3.1 co-transfection.