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. Author manuscript; available in PMC: 2010 Apr 13.
Published in final edited form as: Biomacromolecules. 2009 Apr 13;10(4):756–765. doi: 10.1021/bm801199z

Figure 4. Cargo molecules must be localized in the same endolysosome as core-shell particles for cytosolic delivery to occur.

Figure 4

DC2.4 cells were incubated with calcein alone for 1 hr at 37 °C in medium containing 10% FBS, washed, and then incubated a second hour at 37°C with serum-containing medium alone (A), with calcein and 25 µg/mL PDEAEMA CSPs simultaneously in serum-containing medium (B), or with CSPs alone in serum-containing medium (C). Cells were washed after the second hr and imaged live at 37°C by CLSM. Images show fluorescence overlays of calcein (green) and Cy5-labeled particles (red). Scale bars 20 µm.