Skip to main content
. Author manuscript; available in PMC: 2010 Apr 13.
Published in final edited form as: Biomacromolecules. 2009 Apr 13;10(4):756–765. doi: 10.1021/bm801199z

Figure 7. pH-sensitive core-shell particles enable cytosolic siRNA delivery and gene knockdown.

Figure 7

(A, B) CLSM images of labeled siRNA (blue) and CSP (red) delivery in DC2.4 cells. DC2.4 cells were incubated for 1 hr in complete medium (10% FBS) with siRNA adsorbed to control PMMA CSPs (A) or pH-responsive DEAEMA CSPs (B), washed, and imaged live at 37°C. Scale bars in A and B 10µm. (C, D) CLSM images of labeled siRNA (blue, Cy5-labeled sense strand; red, Cy3-labled anti-sense strand) and CSP delivery in BSC-40 epithelial cells. Epithelial cells were incubated in serum-containing medium with siRNA adsorbed to PMMA CSPs (C) or DEAMA CSPs (D) for 1 hr, washed, and imaged live at 37°C. Scale bars in C and D 20 µm. (E) RT-PCR analysis of Cyclophilin B mRNA downregulation at 24 hrs following siRNA delivery into BSC-40 cells via DharmaFECT1® transfection reagent (siRNA/Lipid, 4 hrs) or siRNA adsorbed to CSPs (siRNA/DEAEMA CSPs, 1hr), relative to untreated control cells.