Figure 5.

Salmonella Choleraesuis induced caspase-1 dependent cytotoxicity in RAW 264.7 cells was impaired in ∆crp mutants. Bacteria grown under conditions that induce SPI-1 T3SS were co-cultured at a MOI of ~ 50 with RAW 264.7 cells treated with 0.5% DMSO carrier (Vehicle) or with 50 μM caspase-1 inhibitor Ac-YVAD-CMK (Casp-1). Cell death was assessed at 6 hpi by measuring the release of lactate dehydrogenase (LDH). WT, wild type Salmonella Choleraesuis; CP327 and CP359, crp gene mutants; Comp., CP327 complemented with pTCRP plasmid. The results are from three independent experiments of at least triplicate determinations. Error bars are SEM. **p < 0.01; ***p < 0.001; n.s.: no significant difference from each other (ANOVA).