Table II.
Oligonucleotide primers used and plasmids constructed in this study.
| Primer | Sequences (5′ → 3′)a | Plasmid |
|---|---|---|
| sopB P1(F) | CAGAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGCAAATACAGAGCTTC | pTSopB |
| sopB P2(R) | CCGAAGCTTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCAGATGTGATTAATGAAGA | |
| sipB P1(F) | CAGAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGGTAAATGACGCAAGTAGCATTAG | pTSipB |
| sipB P2(R) | CCGCTGCAGTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCTGCGCGACTCTGGCGCAGAATAAAACG | |
| crp P1(F) | CAGAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGGTGCTTGGCAAACC | pTCRP |
| crp P2(R) | CCGAAGCTTTTACAGATCCTCTTCAGAGATGAGTTTCTGCTCACGGGTGCCGTAGACGA | |
| crp P3(F) | AATGCCGGATCCTGGACAGCAAGCGAACCGGAATTGCC | p705CRP |
| crp P3(R) | CGGGGATCCTCACACAGGAAACAGCTATGAC |
a
The Myc-tag sequences are shown in italics. Restriction sites are underlined. Stop codons are shown in bold italics. Sequences of partial neomycin promoter of Tn5 transposon are shown in bold.