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. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: Virology. 2009 Jul 28;392(1):137–147. doi: 10.1016/j.virol.2009.06.051

Figure 7. Infection with HSV-2 encoding HA-tagged UL13 alters the nuclear lamin network.

Figure 7

HeLa cells were left uninfected (A, E, I) or were infected with HSV-2 333-UL13-HA at an MOI of 5. At 18 hr post-infection, cells were fixed, stained with anti-HA antibody (green) and anti-lamin antibodies (red). Antibodies used were (A–D) monoclonal lamin A/C antibody, (E–H) polyclonal lamin A/C antibody, or (I–L) polyclonal lamin B1 antibody. Cells were analyzed by confocal microscopy and the anti-HA and anti-lamin images were merged. All images were obtained at the same microscope settings, and representative images are shown. Abnormalities in the lamin staining pattern include decreased lamin staining, arrowhead; invagination, arrow; increased intranuclear lamin staining, open block arrow; and discontinuous staining at the nuclear rim, asterisk. (M) Fifteen random fields of infected cells and three random fields of mock-infected cells were analyzed and lamin staining intensity was ranked as high, medium or low by a masked observer. Graph shows the mean ± SD.