Figure 2.
Standard curve showing the direct relationship between the magnitude of the 571 nm absorption peak and the enzyme concentration using the Amplex Red reagent. Glucose oxidase - open squares, lactate oxidase - solid diamonds. Standard error bars are shown. The abbreviation A.U. refers to Absorbance Units. Note that, compared to the o-dianisidine assay, the Amplex Red assay is better adapted to measure low concentrations of oxidase enzymes.