Figure 4.

(A) The steady-state level of Ho-LacZ activity is compared with that of LacZ in a series of ubc mutants and their congenic wild types. Ho is stabilized in ubc2^rad6, ubc3^cdc34, and ubc9 mutants. (B) The degradation of Ho was followed by pulse–chase and immunoprecipitation in ubc2^rad6 and ubc3^cdc34 mutants and their isogenic wild types. Graph shows the amount of protein remaining at each time point. (C) Degradation of Ho-LacZ in a rad18 mutant and its congenic wild type. (D) Steady-state level of Ho-LacZ compared with that of LacZ in these strains after 18 h induction. Ho is stabilized in the rad18 mutant.