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Ratio of fluorescence intensities at two emission wavelengths (RtHb=F500∕F570) as a function of StO2 (a) for varying scattering coefficient, while the total hemoglobin concentration was fixed at 31 μM and (b) for varying total hemoglobin concentration, while the scattering coefficient was fixed at 200 cm−1. The excitation wavelength was 414 nm.
