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Serum IgG specific HBsAg titers after single dose immunization of pigs intramuscularly with HBsAg, HBsAg formulated with PCPP, and intradermally using microneedles coated with HBsAg and PCPP formulation (seven animals per group, 20 μg of HBsAg, PCPP formulations contained 66 μg of the polymer). *, Significantly higher than in both intramuscular groups. P < 0.05 (ANOVA). **, Significantly higher than in a nonadjuvanted intramuscular group. P < 0.05 (ANOVA).
