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Values of 13C=O NMR chemical shifts in water at 2 °C (blue), 25 °C (orange), 60 °C (magenta). Host values are filled circles. Values for mutants 1 tLeu-Ala9XxxAla9-tLeu are coded as follows: a. open triangles: Xxx = Nva; filled triangles: Xxx = Val. b. Filled squares: Xxx = Nle; open squares: Xxx = Leu; filled diamonds: Xxx = Ile. The closed-line fits to host data that appear in a. are repeated in b. Each shift is site-coded within each peptide by a specific 13C/12C ratio.
