FIG. 5.

Cldn5 mRNA expression (n = 3–4 mice/group). A) Cldn5 expression remained constant from birth through Postnatal Day 12 and then increased at Postnatal Days 16 and 20 (*P < 0.05 compared with Postnatal Day 0). B) Cldn5 expression was decreased in 8-day-old (d8) and adult Etv5^−/− mice (*P < 0.05 compared with WT mice at the same age). To adjust for a germ cell dilution effect that occurs with testicular development, Cldn5 expression was determined relative to Gata6, a gene expressed in Sertoli cells but not in germ cells [22].