FIG. 3.

Semiquantitative PCR analysis of candidate imprinted genes. Samples were analyzed as described in the text. A) Expression of variant 1 and/or variant 2 for INPP5F. INNP5F_V2 has increased expression in BP compared with PRT samples in all tissues tested. B–D) Combined results for PEG10, PEG3, and SNRPN showing expression from the BP samples but lack of or highly reduced expression from the PRT sample, a pattern supportive of paternal expression. Additionally, the PCR results confirm the microarray data. E) Expression of PPP1R9A showing greater expression in the PRT than the BP placental sample but similar BP and PRT expressions in all other tissues. F) RPL18 was used as the control baseline transcript. Two independent RNA pools were used to generate these results. BR, brain; FIB, fibroblast; LIV, liver; PLA, placenta. *Both cDNA pools behaved similarly.