Table 2.
Sequence conservation in serpin core and RCL between D. melanogaster and D. grimshawi
| Serpin |
P1/P1' mel |
P1/P1' gri |
Alignment Score % Serpin core |
Alignment Score % RCL |
| Spn27A | K/F | K/F | 70 | 87 |
| Spn28Dc | S/G | S/G | 45 | 76 |
| Spn31A | - | - | 67 | 80 |
| Spn42Da | A/S | A/S | 66 | 69 |
| Spn42Db | K/G | R/S | 46 | 49 |
| Spn42Dc | M/M | M/S | 52 | 75 |
| Spn43Aa | M/S | M/S | 80 | 95 |
| Spn43Ab | - | - | 77 | 90 |
| Spn43Ac | L/S | L/S | 41 | 72 |
| Spn43Ad | - | - | 53 | 46 |
| Spn47C | - | - | 41 | 44 |
| Spn55B | R/M | R/M | 76 | 94 |
| Spn77Ba | K/A | K/S | 58 | 72 |
| Spn85F | - | - | 68 | 96 |
| Spn88Ea | S/A | S/A | 55 | 84 |
| Spn100A | - | - | 62 | 67 |
The putative proteinase cleavage sites (P1/P1') of inhibitory serpins rarely change between the D. melanogaster and D. grimshawi orthologues and the RCL sequence is more strongly conserved than the serpin core. The RCL of non-inhibitory serpin-folds tends to be weakly conserved, with three exceptions, Spn31A, Spn43Ab and Spn85F. The putative RCL of the serpin-related fold, Spn100A, is conserved slightly more strongly than the remainder of the protein.