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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Mol Microbiol. 2009 Sep 2;74(1):209–226. doi: 10.1111/j.1365-2958.2009.06862.x

Figure 2. Effects of chemical agents and the RR → KK mutation on membrane binding and transport of pre-SufI.

Figure 2

All gels in this figure are anti-SufI immunoblots. (A) Effect of pH on binding and transport efficiency. Shown is the effect of pH on pre-SufI binding (top), and on transport in the presence of 4 mM ATP with an ATP regenerating system (middle) or 4 mM NADH (bottom). The data were quantified as in Fig. 1C: (blue) precursor bound to pTat IMVs, (red) lipid-bound precursor, (green) translocon-bound precursor, (black, solid) precursor transported with NADH, and (black, open circles) precursor transported with ATP (n = 3). (B) Effect of KCl concentration on binding and transport efficiency. Key as in A (n = 3). (C) Effect of KK substitution for the RR motif (pre-SufI-KK-CCC) on pre-SufI binding and transport efficiency (Tr) (n = 3). (D) Effect of urea concentration on binding and transport efficiency. Key as in A (n = 3).