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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Mol Microbiol. 2009 Sep 2;74(1):209–226. doi: 10.1111/j.1365-2958.2009.06862.x

Figure 3. Effect of chemical agents on the membrane binding and transport efficiency of GFP precursors.

Figure 3

The gels in this figure are anti-SufI (B, top) or anti-GFP (all others) immunoblots. (A) Concentration dependence of the binding efficiency of spTorA-GFP and spSufI-GFP. The data were quantified as in Fig. 1C: (dashed) precursor bound to pTat IMVs, (dotted) lipid-bound precursor, (solid) translocon-bound precursor, (blue) spTorA-GFP, and (red) spSufI-GFP (n = 3). The upper band on the anti-GFP immunoblot results from immuno-crossreactivity to a non-GFP protein and was only occasionally observed. (B) Effect of 2 M urea (U), 1 M KCl (K) and 2 M urea + 1 M KCl (U + K) on the membrane binding efficiency of pre-SufI (red), spTorA-GFP (green) and spTorA12-GFP (blue). The plot shows averaged data (n = 3) for ΔTat (hatched) and pTat (solid) IMVs. The precursor bound to pTat IMVs is considered the control (100%). (C) Effect of the magnitude of the PMF on the transport efficiency of spTorA-GFP and spTorA12-GFP. In B and C, 8.3 pmol of spTorA-GFP and spTorA12-GFP were added to the reactions; in B, 3.1 pmol of pre-SufI was added.