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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: J Immunol. 2007 Apr 1;178(7):4194–4213. doi: 10.4049/jimmunol.178.7.4194

FIGURE 11.

FIGURE 11

Anti-CD137 treatment of TNF-α-deficient mice. BL/6 TNF-α−/− mice were injected i.p. with 200 µg of rat IgG or anti-CD137 mAbs 1 × weekly for 3 wk. The following week, the mice were bled and euthanized. Spleen, inguinal LNs, and livers were weighed (a–c), and tissues were processed into single-cell suspensions, stained for CD8 T and CD19-positive B cells, and analyzed by FACS. From FACS frequency plots and microscopic count of viable cells, absolute numbers of liver and LN CD8 T cells were determined (d and e, respectively), and LN and splenic CD19 B cells were determined (f and g, respectively).