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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: J Immunol. 2007 Apr 1;178(7):4194–4213. doi: 10.4049/jimmunol.178.7.4194

FIGURE 5.

FIGURE 5

a, In vivo proliferation of LN CD8 T cells and B220 B cells was conducted by adoptively transferring CFSE-labeled BL/6 CD45.1 congenic spleen cells into wild-type CD45.2 BL/6 mice before treating the mice with five weekly injections of 200 µg of anti-CD137 or rat IgG. FACS analysis was performed at week 6. CD45.1 CD3+CD8+ T cell and CD19+ B cell proliferation was determined by CFSE dilution. Black histograms represent CD137-treated mice, and red histograms represent rat IgG-treated mice. The percentages shown for each histogram represent the frequency of nondividing cells in each gated population denoted by the horizontal bar intersecting the histogram peak. b, Inguinal LN B and T cell proliferation was also determined by BrdU short pulse labeling for 4 h following the third weekly injection of anti-CD137 or rat IgG.