Table 3. Schedule of injections.
| Injection schedule (weeks)a | Dose per antigen (µg)b | Vehicleb |
| 14 | 500 | FCA |
| 12 | 500 | Alum |
| 10 | 250 | FIA |
| 8 | 250 | Alum |
| 6 | 250 | PBSc |
| 4 | 250 | Alum |
Notes FCA, Freund's complete adjuvant; FIA, Freund's incomplete adjuvant; PBS, phosphate-buffered saline.
a
Preceding natural exposure to infection.
b
Adjuvant controls received an equivalent volume of PBS instead of antigen, in combination with vehicle, following an identical schedule to vaccinated animals.
c
Final injection in the Freund's series.