Figure 3. Effects of Tregs on chemokine production during an immune response.

Stg and dtg mice were injected in the footpad with HA peptide (1 µg) together with LPS (0.5–1 µg). Control mice received LPS alone or nothing. Mice were sacrificed at different times after injection, as indicated. (A) dLNs were weighed and (B) CCL2, CCL3 and CCL5 chemokine content after homegenization was determined by ELISA. Shown are the pooled data from two independent experiments, with two to three mice per group in each experiment. (C) BALB/c mice were adoptively transferred with CFSE-labelled 6.5⁺ cells obtained from stg mice without or with equal numbers of 6.5⁺ cells isolated from dtg mice. The mice were immunized as described above. dLN were harvested and CCL2 amounts were determined by ELISA. Data from two pooled out of three independent experiments performed are shown.