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. Author manuscript; available in PMC: 2009 Oct 29.
Published in final edited form as: Biochemistry. 2008 Sep 5;47(39):10324–10332. doi: 10.1021/bi8007565

Fig. 2.

Fig. 2

The engineered ascorbate binding loop in CCP2APX. (A) 2Fo−Fc electron density map contoured at 1.0 σ. Note that the engineered loop centered on Lys33 and Arg 172 which is Asn in WTCCP are very well ordered. (B) A superimposition of CCP2APX/F191 (red) on the APX-ascorbate complex (green). The key components of the ascorbate binding site are the same in both enzymes.