Table 2.

Blocking EIA for deer IgG anti-hepatitis E virus (anti-HEV)

	ODa
Sample Eluate	Blocked with HEVb	Blocked with gelatin	Percent reduction in OD	Dilution of blocking
HTL 55	0.801	0.700	−14	1:50
HTL 58	0.366	0.353	−4	1:50
HTL 29	0.368	0.406	9	1:50
HTL 7	0.173	0.142	−22	1:50
HTL 128	0.196	0.141	−39	1:50
Negative eluate controlc,d	0.087	0.091	4	1:50
Positive eluate controlc,e	0.199	0.594	66	1:104
Negative serum controld	0.075	0.077	3	1:50
Positive serum controle	0.201	0.848	76	1:104

^aOD is the optical density at 405 nm.

^bSamples and controls were blocked with 1 μg/mL of HEV Sar-55.

^cFilters inoculated with 400 μL of deer blood were used for this study.

^dBaseline eluate and sera were used as negative controls.

^ePositive controls were collected six weeks after the immunization.