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. Author manuscript; available in PMC: 2010 Oct 2.
Published in final edited form as: Cell. 2009 Oct 2;139(1):186–198. doi: 10.1016/j.cell.2009.08.033

Figure 3. Altered LTM Formation via Genetic Manipulation of CSW.

Figure 3

(A) Effect of the protein synthesis inhibitor, cycloheximide (CXM+), or vehicle (CXM−) on 24-hr memory after spaced training in flies expressing mutant or wild-type csw in the mushroom body neurons. Memory was reduced in the control group (OK107;+) and in flies overexpressing the wild-type csw (WT) by the inhibitor (CXM +) compared with both groups fed with vehicle (CXM −), but not in csw gof mutants. (B) 24-hr memory after spaced or massed training in control (OK107;+) and transgenic flies expressing mutant and wild-type csw in the mushroom body neurons. Spaced training promoted a higher performance in control (OK107;+) flies compared with the massed training in the same line or the mutant lines (D61Y and E76K). Note that massed training in flies overexpressing wild-type csw produced a similar memory score as spaced training. (C) 24-hr memory after one trial (1 T), or 5 and 10 trials of massed training (5 m and 10 m, respectively) in control (OK107;+) and flies overexpressing wild-type csw in mushroom body neurons (OK107;UAS-csw+), which showed memory enhancement after 10 m compared with all the control bars (OK107;+) or the same lines trained with 1 or 5 trials. The effect of the cold shock (D) or cycloheximide (CXM) (E) on 24-hr memory after 10 trials of massed training in flies overexpressing wild-type csw in mushroom body neurons. Memory enhancement was detected in OK107;UAS-csw+ in the absence of cold shock or CXM compared with the same line untreated or with the control (OK107;+) line with or without treatment. (F) 24-hr memory after 10 trials of massed training in flies expressing wild-type or mutant csw in alpha/beta neurons of the mushroom bodies using the c739-GAL4 driver (c739;UAS-csw) compared with control (c739;+). Memory enhancement was detected in flies expressing wild-type csw compared with the control group or mutant lines. (G) 24-hr memory after 10 trials of massed training compared between flies overexpressing wild-type and phosphatase-dead mutant (R465M) csw in the mushroom body neurons. * indicates WT vs. R465M, Student's t test. Bars, mean ± SEM (N=6). Asterisks indicate p<0.05.