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. Author manuscript; available in PMC: 2010 Feb 20.
Published in final edited form as: Immunity. 2009 Feb 5;30(2):277–288. doi: 10.1016/j.immuni.2008.11.013

Figure 5. DCs are the only cells in antigen injection sites that present antigen and induce T cell lymphokine production.

Figure 5

(A) Representative CD11c versus CD45 staining for cells isolated from the ears of B6 mice injected 12 days earlier with either EαRFP/IFA or OVA/IFA. (B) CFSE dilution of naïve TEa T cells incubated with cells sorted based on the gates shown in (A). The sorted populations were from: OVA/IFA injection sites (B, top rows) or EαRFP injection sites (B, bottom rows). Gates indicate the percentage of TEa cells that proliferated during the culture. Data are representative of three individual experiments. (C) Representative GFP versus CD11c staining of anti-CD11c magnetic bead-enriched cells from antigen injection sites of CD11c-DTR mice 24 hours after PBS (left panels) or DT injection (right panels). Scatterplots on the right show the percentage of DCs in samples from individual mice. (D) Representative intracellular IFN-γ staining of Foxp3− or (E) IL-10 staining of Foxp3+ TEa T cells from CD11c-DTR recipients injected with EαRFP/IFA, then 11 days later with DT (right panels) or PBS (left panels) into the original injection sites. Intracellular staining was performed 24 hours after DT or PBS injection. Scatterplots (far right panels) show the percentage of cytokine-producing TEa T cells from individual mice. (n=7 from 2 pooled experiments). *, P<0.0001; **, P=0.0005; ***, P=0.02.