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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cell Death Differ. 2009 Jun 26;16(10):1362–1371. doi: 10.1038/cdd.2009.79

Figure 5.

Figure 5

Lysosome clustering and acidification of nurse cell remnants. All egg chambers stained with DAPI (blue) and LysoTracker (LT, red). (a-c) Progression of LT labeling in wild-type egg chambers. (a) In early st12, increased LT labeling is observed in clusters around NC nuclei. (b) In late st12, acidification of NC nuclei is indicated by large LT-positive domains (arrow). (c) Large LT-positive domains are also observed in st13 egg chambers (arrow). (d-f) Egg chambers from a GFP gene trap in Cp1 (cathepsinL). (d) In early st12, Cp1-GFP clusters around NC nuclei and partially overlaps with LT. (e) In late st12, most Cp1-GFP remains punctate and LysoTracker is detected in large domains. (f) In st13, no significant overlap is detected between CP1-GFP and LT. (g-i) LT labeling in dor4 homozygous mutants reveals a delay in acidification of NC remnants. LT is punctate in st12 (g) and st13 (h) dor4 homozygous egg chambers. (i) A dor4 homozygous st14 egg chamber shows acidification of some persisting NC nuclei. Egg chambers in a-i are projections of 11-21 consecutive images. Scale bar is 50μm.