Figure 1. Loss of Rts1 causes reduced polar growth.

(A) rts1Δ reduces the polar growth caused by inactivation of septins. Cells of the indicated genotypes were grown overnight to log phase at room temperature and then shifted to 30°C for 4 hours. (B) rts1Δ reduces the polar growth caused by inactivation of kinases that regulate the septins. Cells of the indicated genotypes were grown to log phase. The gin4Δ and gin4Δ rts1Δ cells were grown at room temperature, while the others were grown at 30°C. (C) rts1Δ does not eliminate the G2/M delay caused by elm1Δ. Wild type, elm1Δ, and elm1Δ rts1Δ cells were released from an α factor arrest at 30°C. Samples were collected at 10 minute intervals and levels of the mitotic cyclin Clb2 were detected by Western blotting.