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. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: Biochemistry. 2009 Sep 29;48(38):9122–9131. doi: 10.1021/bi900675v

Figure 3.

Figure 3

Insertion of YFP protein into the N-terminus of ABCG2 did not significantly affect its nucleotide-dependent MTX transport. The MTX uptake assays were carried out, according to the method described in Experimental Procedures, in triplicate with 3 μg of membrane vesicles shown in Figure 2B and 2C in the presence of varying concentrations of ATP or dATP and 10 mM DTT. A. ATP-dependent MTX transport by YFP/ABCG2. B. dATP-dependent MTX transport by YFP/ABCG2. C. ATP-dependent MTX transport by YFP/ABCG2 + E211Q. D. dATP-dependent MTX transport by YFP/ABCG2 + E211Q.