Figure 9.

Examples of effects of MnPN chemical activation and inhibition on discharge of PF/LH sleep-related neurons. A, B, Effects of microdialytic perfusion of the MnPN with 1 mm l-glutamate and 20 μm muscimol, respectively. The arrows indicate the beginning (white arrow) and the end (black arrow) of drug perfusion. Expanded tracings from the sections marked by vertical bars show neuronal discharge in QW episode preceding NREM sleep before (a₁), during (a₂), and after (a₃) microdialysis of l-glutamate and in NREM sleep before (b₁), during (b₂), and after (b₃) microdialysis of muscimol. Note an increase of neuronal discharge during l-glutamate perfusion and a decrease during muscimol perfusion.