A. Chromatogram of substrate Abz-γ-D-Glu10-Dnp.
B. Chromatogram of reaction products after incubation of substrate Abz-γ-D-Glu10-Dnp with CapD.
C. Chromatogram of reaction products after incubation of substrates Abz-γ-D-Glu10-Dnp and L-Ala-D-iGlu-m-DAP with CapD. MALDI-TOF analysis of reaction products is provided in Supplemental Tables 1 and 2.
D. Comparison of product yields, expressed as relative abundance (%), for CapD catalysis in panels B and c.
E. Tandem mass spectrometry experiment in the positive reflector mode of m/z 897.35, the CapD transpeptidation product L-Ala-D-iGlu-(Abz-[γ-D-Glu]3)-m-DAP. Daughter ion signals and structural predictions are listed in Table 2.
F. Structural schematic and corresponding b- and y-ions derived from m/z 897.35.