Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
C-Tpr import into the nucleus is cytosol, temperature and energy dependent and is blocked by WGA. Digitonin-permeabilized NRK cells were incubated with 8 pmoles of Cy5-C-Tpr for 20 min at 30°C (panel A) or 4°C (panel B) in the presence of cytosol and an energy-regenerating system. Panel C: 8 μg WGA was added. Panel D: the ATP regenerating system was replaced by an ATP-depleting system, 0.8885 U hexokinase + glucose. Samples were visualized by confocal microscopy.
