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Comparative analysis of noncanonical (top) and unclassified (bottom) Wnt ligands in hepatocellular carcinoma cell lines. Noncanonical and unclassified Wnt ligands were tested for expression by RT-PCR assay. Total RNAs were extracted from cell lines and used to detect gene expression by RT-PCR assay. See Figure 1 for GAPDH loading control.
